Anticancer Effect of Ginger Extract against Pancreatic Cancer Cells Mainly through Reactive Oxygen Species-Mediated Autotic Cell Death (original) (raw)
Fig 2
Effect of SSHE on the features of apoptosis in Panc-1 cells.
(A) Mitochondrial membrane potential. Panc-1 cells were treated with vehicle alone, 100 μg/ml SSHE or 200 μg/ml SSHE for 20 h and then subjected to the JC-1 assay. Healthy cells with functional mitochondria that contain red JC-1 J-aggregates and apoptotic or unhealthy cells with collapsed mitochondria that contain mainly green JC-1 monomers were detected by cytometry. (B) Annexin V/PI staining. Panc-1 cells were treated with vehicle alone, 100 μg/ml SSHE or 200 μg/ml SSHE for 24 h and then subjected to annexin V/PI staining. (C) Effect of zVAD-fmk (zVAD) on SSHE-induced cell death. Panc-1 cells were incubated for 42 h. Cell viability was assessed by the MTT assay. Bars; SD. (D) Caspase-3 activation. Panc-1 cells were incubated with 200 μg/ml SSHE for various time periods or treated with 10 μM pifithrin-μ and 100 ng/ml TRAIL for 60 h (served as positive control). The cell lysates were subjected to Western blot analysis with anti-caspase 3 antibody.