Anticancer Effect of Ginger Extract against Pancreatic Cancer Cells Mainly through Reactive Oxygen Species-Mediated Autotic Cell Death (original) (raw)
Fig 4
Effect of SSHE on the morphological features of Panc-1 cells.
(A) Phase contrast. Panc-1 cells were treated with vehicle alone or 100 μg/ml SSHE for 40 h. Bars; 100 μm. (B) LC-3 puncta. Panc-1 cells transfected with pCMX-SAH/Y145F-LC3B-GFP vector were treated with vehicle alone or 100 μg/ml SSHE for 24 h and were observed under a confocal laser microscope. Bars; 20 μm. (C) Double staining with MitoTracker and anti-LC3 antibody. Panc-1 cells were treated with vehicle alone, 100 μg/ml or 200 μg/ml SSHE for 24 h, stained with 100 nM of MitoTracker Red for 10 min, fixed, and then processed for LC3 immunostaining. Bars; 20 μm. (D) Ultrastructure. Panc-1 cells treated vehicle alone or with 200 μg/ml SSHE for 28 h were processed for TEM analysis. Original magnification, x2,500. PC, perinuclear space.