Interactions between Skeletal Muscle Myoblasts and their Extracellular Matrix Revealed by a Serum Free Culture System (original) (raw)
Fig 3
SDS-PAGE and Western blotting indicates preservation of matrix proteins.
(A) After PLA2 decellularisation ECM proteins were solubilised, resolved on a 7.5% SDS-PAGE and visualized by Coomassie Blue staining: PLA2 decellularised matrix (lane 3) and collagen 1 (lane 2). (B) Muscle extracts (untreated and PLA2 treated) were resolved on 4–15% gradient gels and membranes were probed with antibodies against collagen I, collagen VI, fibronectin, and perlecan (all rabbit polyclonals) and laminin α2 (rat, clone 4H8-2). Secondary antibodies were anti-rabbit or anti-rat conjugated to HRP, and blots were visualized using ECL. C: untreated muscle extract, PLA2: decellularise muscle extract.