Mouse Models of Diet-Induced Nonalcoholic Steatohepatitis Reproduce the Heterogeneity of the Human Disease (original) (raw)
Fig 4
Effects of MCD diet and Western diet on liver cell death, inflammation and oxidative stress.
(A). TUNEL assay and Western blot for caspase-2 and cleaved PARP; (B). qRT-PCR of macrophage markers (F4/80 and YM-1) and TNF-α (left). Immunohistochemistry for F4/80 and YM-1: Representative photos and morphometry (right). (C). qRT-PCR analysis of anti-oxidant enzymes (top) and immunohistochemistry plus morphometry for 4-hydroxynonenal (4-HNE) in representative mice (lower). Results normalized to chow-diet fed mice and graphed as mean±SEM. *<0.05 and **<0.005, control versus experimental diet; ##<0.005, MCD versus Western diet.