Additive Regulation of Adiponectin Expression by the Mediterranean Diet Olive Oil Components Oleic Acid and Hydroxytyrosol in Human Adipocytes (original) (raw)
Fig 3
Effect of combined treatment with HT and OA on TNF-α-induced inhibition of adiponectin protein release and expression.
SGBS cells were pretreated with either HT, OA or cotreated with HT + OA before 10 ng/mL TNF-α stimulation for 24 h. (A) Adiponectin in the culture medium was determined by ELISA, and expressed as percent of unstimulated control (CTL). (B) Adiponectin intracellular protein levels were determined by Western analysis using antibodies against adiponectin. Western analysis under reducing and denaturing condition here reveals the 30 kDa adiponectin monomer. Adiponectin expression was normalized to β-actin, and expressed as percent of unstimulated control (CTL). Data are means ± SD (n = 3). #p<0.05 versus CTL. *p<0.05 versus TNF-α alone. †p<0.05 versus each compound + TNF-α.