Microbial Population Differentials between Mucosal and Submucosal Intestinal Tissues in Advanced Crohn's Disease of the Ileum (original) (raw)
Fig 2
Influence and effect of rRNA gene copy number on the relative abundance of bacterial populations.
Conversion of data to relative percent of population based on the number of 16s rRNA operons in each organism, thereby representing the number of bacteria or genomes, had a major effect on the composition of the bacterial communities within the mucosa and submucosa. Certain bacteria are either over or under represented when a 1:1 ratio is assumed per normal bioinformatics. Similar disparities were found at all bacterial divisions. Legend: Fasta Count: Relative abundance based on the assumption that each rRNA sequence alignment is equivalent to a single bacterial genome/cell as used in most bioinformatics analyses. rrn Operon Count: relative bacterial abundance based on the average number of rRNA operons in each bacterial cell (fasta count/rrn operons) thereby more accurately reflecting bacterial prevalence.