Dopaminergic Toxin 1-Methyl-4-Phenylpyridinium, Proteins α-Synuclein and Glia Maturation Factor Activate Mast Cells and Release Inflammatory Mediators (original) (raw)
Fig 6
Human mast cells express GMF.
Cytospin smears of cultured human mast cells were analyzed first for mast cell specific tryptase using mouse anti-human mast cell tryptase by ICC (n = 3). Mast cells were then analyzed for the expression of GMF using GMF polyclonal antibody and GMF monoclonal antibody (n = 3). GMF ICC with polyclonal antibody was carried out using ImmPRESS reagent anti-rabbit Ig peroxidase, and tryptase and another GMF ICCs were carried out with monoclonal antibodies using ImmPRESS reagent anti-mouse Ig peroxidase. ImmPACT DAB peroxidase substrate kit was used for both IHCs. Presence of brown color indicates the expression of GMF in human mast cells. Isotype matched control antibodies did not show positive reaction. Original magnification = 400x.