Dopaminergic Toxin 1-Methyl-4-Phenylpyridinium, Proteins α-Synuclein and Glia Maturation Factor Activate Mast Cells and Release Inflammatory Mediators (original) (raw)
Fig 8
Mouse mast cells and mouse astrocyte co-culture.
Mouse mast cells and mouse astrocytes were co-cultured in tissue culture plate to evaluate the effect of GMF (100 ng/ml) stimulation individually on either mast cells or astrocytes or in a co-culture system consisting of both mast cells and astrocytes in the same well (n = 4). Cells were incubated with GMF for 24 h and the supernatant media were collected by centrifugation. TNF-α level was measured by ELISA. More TNF-α release was observed from astrocyte and mast cell co-culture system than released either from astrocyte alone or mast cells alone upon incubation with GMF. # = compared to astrocyte + GMF or mast cells + GMF. *p<0.05, = compared to respective unstimulated control cells, ANOVA and Tukey-Kramer.