Dopaminergic Toxin 1-Methyl-4-Phenylpyridinium, Proteins α-Synuclein and Glia Maturation Factor Activate Mast Cells and Release Inflammatory Mediators (original) (raw)
Fig 9
GMF and MPP+ induce IL-33 or CD40 expression in mouse astrocytes as determined by flow cytometry.
Astrocytes were incubated with GMF (50 ng/ml) or MPP+ (25 μM) for 72 h at 37°C in vitro and the expression of IL-33, CD40 or CD40L were analyzed by flow cytometry using monoclonal anti-mouse IL-33-phycoerythrin or monoclonal anti-mouse CD40/TNFRSF5-APC (n = 3). GMF and MPP+ increased IL-33 expression (A, B; green color) when compared to un-treated control cells. Incubation of mouse astrocytes with GMF also induced the expression of (C) CD40, and to some extent (D) CD40L.