Increased Notch Signaling Enhances Radioresistance of Malignant Stromal Cells Induced by Glioma Stem/ Progenitor Cells (original) (raw)
Fig 3
Notch1/Hes1 signaling pathway activation enhances the radiation resistance of ihBTC cells.
A-B, Expression of Notch1 and Jag1 mRNA of SU3-5R and ihBTC2 cells increases after radiation (P<0.05). C-D, Notch1 and NICD protein of SU3-5R and ihBTC2 increased after radiation (P<0.05). E, GSI significantly inhibited the up-regulation of Hes1 mRNA from ihBTC2 cells compared to the untreated control group. (P<0.01). F, Phosphorylated Akt and Bcl-2 protein expression of ihBTC2 cells increased significantly after radiation (P<0.05). The up-regulation of these proteins in ihBTC2 cells induced by radiation was inhibited by GSI (P<0.05).