Increased Notch Signaling Enhances Radioresistance of Malignant Stromal Cells Induced by Glioma Stem/ Progenitor Cells (original) (raw)
Fig 4
Radiosensitivity of ihBTC2 is improved by inhibiting the Notch signaling pathway.
A-B, The cell proliferation (A) and the colony forming ability (B) of the radiation combined with GSI group is decreased compared to the radiation group (P<0.05). C, The SF2 of the untreated and GSI-treated ihBTC2 cells were 0.57±0.05 and 0.75±0.03 (P<0.05), respectively. D-E, Compared to the radiation group, the caspase-3/7 activity of the radiation combined with GSI group was significantly increased after 48 hours (D) and 72 hours (E). F, Trypan blue staining indicated that the dead cell rate of the radiation combined with GSI group was significantly higher compared to the radiation group after 96 hours (P<0.05). G, After 24 hours, the Annexin V staining of the blank, GSI, radiation and radiation combined with GSI group were 0.22%±0.04%, 0.29%±0.04%, 4.60±0.43% and 11.68±0.52%, respectively.