Molecular Genotyping of Giardia duodenalis Isolates from Symptomatic Individuals Attending Two Major Public Hospitals in Madrid, Spain (original) (raw)
Fig 1
Evolutionary relationships among assemblages of G. duodenalis at the GDH locus inferred by a neighbour-joining analysis of the nucleotide sequence covering a 359-bp region (positions 103 to 461 of GenBank accession number L40508) of the gene.
GenBank accession numbers are provided for each sequence used. The bootstrap consensus tree was inferred from 1,000 replicates. Branches corresponding to partitions that were reproduced in less than 50% of bootstrap replicates are collapsed. The bootstrap values are indicated at the branch points. The evolutionary distances were computed using the Kimura 2-parameter method. The rate variation among sites was modelled with a gamma distribution (shape parameter = 2). Spironucleus vortens was used as outgroup taxa.