Differential Expression of the Activator Protein 1 Transcription Factor Regulates Interleukin-1ß Induction of Interleukin 6 in the Developing Enterocyte (original) (raw)

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Fig 3

Interleukin 1β induction of JNK and p38 phosphorylation in immature H4 and mature NCM-460 enterocytes.

Western analysis of JNK and p38 phosphorylation in immature H4 enterocytes (A) and mature NCM-460 enterocytes (B) in response to IL-1β. Cells were grown in 10 cm dishes to 90% confluence, incubated in low serum media for 3h followed by exposure to IL-1β (0.5 ng/ml). Cells were harvested at the indicated time points and lysates (15ug) run on SDS PAGE (4–12%) and blotted with the indicated antibodies. Bands were quantitated using quantity 1 software. Molar phosphorylation, calculated as the ratio of the density of the phosphorylated bands to the unphosphorylated bands are presented (lower panels). A representative experiment of 3 separate experiments is presented.

Fig 3

doi: https://doi.org/10.1371/journal.pone.0145184.g003