Overexpression of microRNA-99a Attenuates Cardiac Hypertrophy (original) (raw)
Fig 2
The expression of miR-99a and mTOR in NMVMs upon hypertrophic stimuli.
A. Expression of mTOR and ANP in NMVMs under Ang II stimulation assessed by western blotting analysis. B. ANP expression was up-regulated at least 2.68-fold upon Ang II stimulation. C. Increased mTOR expression was observed as early as 1 hour after Ang II stimulation (increased about 1.60-fold), and lasted for at least 24 hours. D. Expression of mTOR and ANP in NMVMs under ISO stimulation assessed by western blotting analysis. E. mTOR expression was increased under ISO stimulation. F. ANP expression was up-regulated upon ISO stimulation. G. MiR-99a expression was increased about 2.00-fold after ISO stimulation. H. MiR-99a was increased at least 2.00-fold upon Ang II stimulation. Each experiment was repeated more than 3 times. *, p<0.05.