Release of Matrix Metalloproteinases-2 and 9 by S-Nitrosylated Caveolin-1 Contributes to Degradation of Extracellular Matrix in tPA-Treated Hypoxic Endothelial Cells (original) (raw)
Fig 4
Caveolin-1 participated in the secretion of MMP2 and 9.
A) MMP2 and 9 existed in the insoluble fraction P3 of the media of cells treated with OGD+tPA (O+t). B) Western blot analysis showed that Cav-1 siRNA effectively knocked down Cav-1 protein expression in bEND3 cells. Experiments were repeated three times and exhibited the same results. C) Knockdown of Cav-1 with siRNA abrogated OGD+tPA (O+t)-induced existence of Cav-1 (upper panel) and MMP2 and 9 (bottom panel) in P3 in the media. Experiments were repeated three times and exhibited the same results. Std: human MMP2 and 9 standards. D) Cav-1 siRNA recovered the same levels of MMP2 and 9 in the media of cells treated with OGD+tPA (O+t) and in the control (Ctrl), OGD, and tPA treatments. Upper panel: representative zymograms; Bottom panel: band intensity was quantitated and the data were expressed as mean ± SD, ANOVA, N = 3. Std: human MMP2 and 9 standards.