Identification of VPS13C as a Galectin-12-Binding Protein That Regulates Galectin-12 Protein Stability and Adipogenesis (original) (raw)
Fig 2
Galectin-12 and VPS13C are co-upregulated during adipocyte differentiation.
Expression was assayed by quantitative real-time RT-PCR for mRNA levels (A and B) and by immunoblotting for protein levels (C and D) in subconfluent 3T3-L1 fibroblasts, or at different time points of adipocyte differentiation. Adipocyte differentiation was induced at day 0, when cells were three days post confluence, following an established adipogenic regimen [22]. Bar graphs present data (means ± s.e.) from three experiments.