Promotion of Intestinal Epithelial Cell Turnover by Commensal Bacteria: Role of Short-Chain Fatty Acids (original) (raw)
Fig 6
Promotion of the development of intestinal organoids by SCFAs.
(A) Mouse intestinal organoids were incubated in the absence (–) or presence of either 0.5 mM acetate, 0.5 mM propionate, 0.5 mM butyrate, SCFAs (mixture of 0.5 mM acetate, 0.5 mM propionate, and 0.5 mM butyrate), 50 mg/ml EGF, 0.5 mM CPC (GPR41 agonist), or 5 μM 4-CMTB (GPR43 agonist) for 4 days. The organoids were then examined by light microscopy. Scale bar, 100 μm. (B and C) Organoid area (B) and the number of buds per organoid (C) determined for cultures treated as in (A). Data are means ± SE from three separate experiments, with 30 organoids being examined for each condition in each experiment. *P < 0.05, **P < 0.01 (ANOVA and Tukey’s test).