Promotion of Intestinal Epithelial Cell Turnover by Commensal Bacteria: Role of Short-Chain Fatty Acids (original) (raw)
Fig 8
Importance of MEK-ERK signaling in the SCFA-dependent development of intestinal organoids.
(A) Mouse intestinal organoids were stimulated (or not) with 0.5 mM acetate, 0.5 mM propionate, 0.5 mM butyrate, or SCFAs (mixture of 0.5 mM acetate, 0.5 mM propionate, and 0.5 mM butyrate) for 20 min, after which they were lysed and subjected to immunoblot analysis of phosphorylated and total forms of ERK. (B and C) Mouse intestinal organoids were incubated in the absence (–) or presence of SCFAs (mixture of 0.5 mM acetate, 0.5 mM propionate, and 0.5 mM butyrate) with or without MEK inhibitor (10 μM U0126) for 4 days. Organoid area (B) and the number of buds per organoid (C) determined for cultures. Data are means ± SE from three separate experiments, with 30 organoids being examined for each condition in each experiment. **P < 0.01 (ANOVA and Tukey’s test).