cDNA Library Enrichment of Full Length Transcripts for SMRT Long Read Sequencing (original) (raw)
Fig 2
Transcription start and end site enrichment using the TeloPrime Full-Length cDNA amplification kit (Lexogen).
(A-C) Superimposed density plots of the PacBio Iso-Seq alignment coordinates against the 5’ and 3’ ends of their targets. TeloPrime cDNA libraries (red bars), SMARTer cDNA libraries (light blue bars), overlay of the two protocols (brown bars). (A) 1–2 kb size fraction; (B) 2–3 kb size fraction; (C) 3–6 kb size fraction. 10 kb around the annotated gene start and end coordinates are shown on the x-axis. Vertical green dashed lines highlight annotated target start and end sites.