Bioprinted 3D Primary Liver Tissues Allow Assessment of Organ-Level Response to Clinical Drug Induced Toxicity In Vitro (original) (raw)
Fig 3
Histological characterization of 3D liver tissues.
A) A macroscopic image of a 3D liver tissue housed in a 24 well transwell. B) H&E staining of a tissue cross-section; compartmentalization between the parenchymal and non-parenchymal fractions can be readily visualized (dashed line). C) ECM deposition assessed by Masson’s trichrome staining. D) IHC staining of the parenchymal compartment for E-cadherin (Green) and Albumin (red). E) IHC staining for CD31 (red) and desmin (green) to assess organization of the endothelial cells and the presence of quiescent hepatic stellates in the non-parenchymal compartment. F) IHC staining for desmin (green) and α-SMA (red) to assess stellate cell activation. White arrows indicate quiescent stellates in the tissue interior that stain positive for desmin and negative for α-SMA. Cells at the tissue periphery stain positive for α-SMA (white arrowhead), suggesting they have a more activated phenotype. G) Oil-red O staining of 3D liver tissue cryosections to measure lipid storage. H) PAS staining to identify glycogen granules. DAPI was utilized to stain the nuclei of the cells in all of the IHC staining samples (Blue). Scale bars in the lower right hand corner of images are 25μm (B-D, G-H) or 50μm (E, F).