Intestinal Microbiota Promotes Psoriasis-Like Skin Inflammation by Enhancing Th17 Response (original) (raw)
Fig 4
Absence of microbiota or ATB treatment decreases the percentage of γδ T cells and Th17 cells in spleen or axillary lymph nodes of IMQ-treated mice.
Cells from spleen or axillary lymph nodes were isolated and either analyzed for surface γδTCR or intracellular RORγt or stimulated in vitro for 8 h by PMA and Ionomycin, the last 4h in the presence of Brefeldin A and Monensin, and analyzed for intracellular IL-17A and IFNγ production by flow cytometry. Cells were first gated for live cells and CD3+ and subsequently on γδTCR+, RORγt+ or IL-17+ and INFγ+ cells as shown on the (a) example of gating strategy. These results from one representative experiment (n = 5–8 mice per group) out of three independent experiments with (b) GF versus CV mice or with (c) ATB-treated versus control mice are quantified in the graphs. Statistical significance was determined by unpaired Student t test; *p < 0.05, **p < 0.01.