Demonstration of Protein-Based Human Identification Using the Hair Shaft Proteome (original) (raw)
Fig 4
Hair shaft proteomic profile in modern and archaeological samples.
A) Absolute protein abundance from all datasets corresponding to a cohort of European-American subjects (EA2, subjects 1 to 19) and archaeological subjects (S1 to S6) was measured (www.thegpm.org) and collated. Proteins that appeared in proteomic datasets of 15% or more of the subjects (n = 401) were aligned as a paralogous neighbor-joining tree in order to cluster detected proteins with higher levels of homology (www.uniprot.org.). The neighbor-joining tree based on protein paralogy is aligned on the vertical and subjects on the horizontal. Protein abundance is indicated by conditional formatting (maximum value = yellow, minimal value = black). B) The function of individual proteins was obtained (www.uniprot.org) and collated for both modern (EA2, 1 to 19) and archaeological (S1 to S6) hair shaft samples (categories = structural, metabolism, protein and RNA regulation, membrane proteins, and miscellaneous). The relative abundance of the different protein classes is indicated by area. The size of each circle is proportional to the relative abundance of total detected peptides in each sample class.