Endothelial-Mesenchymal Transition in Vascular Calcification of Ins2Akita/+ Mice (original) (raw)
Fig 2
Limiting Sox2 in endothelium decreases EndMTs and calcification in Ins2 Akita/+ aortas.
(a-b) Decreased expression of Sox2, stem cell and mesenchymal markers in aortas of Cdh5 Cre Sox2 Flox/wt Ins2 Akita/+, as shown by real-time PCR (a) and immunoblotting (b). VE-cad:VE-cadherin. (c-d) Total aortic calcium and aortic Alizarin red staining of Cdh5 Cre Sox2 wt/wt Ins2 +/+ and Cdh5 Cre Sox2 Flox/wt Ins2 +/+, Cdh5 Cre Sox2 wt/wt Ins2 Akita/+ and Cdh5 Cre Sox2 Flox/wt Ins2 Akita/+ mice. ***p<0.001.