Definition of Human Epitopes Recognized in Tetanus Toxoid and Development of an Assay Strategy to Detect Ex Vivo Tetanus CD4+ T Cell Responses (original) (raw)
Fig 7
AIM Assay detects tetanus-specific CD4+ effector memory T cell expansion.
(A) Representative flow cytometry plots of CD4+CD25+OX40+ T cells gated on CD45RA and CCR7 expression for central memory (CD45RA- CCR7+), effector memory (CD45RA- CCR7-), naïve (CD45RA+ CCR7+), and TEMRA (CD45RA+ CCR7-) cells after 24 hours of TT-megapool stimulation for a donor originally primed with DTwP vaccine and not boosted (no boost) or after Tdap booster vaccination (boost). The CD4+ cell response to TT-megapool (B) or TT whole protein (C) shows a significant increase after boosting of the effector memory population (p = 0.009 and p = 0.017 respectively) and concomitant decrease of the central memory population (p = 0.028 and p = 0.006 respectively). Two-tailed Mann-Whitney test. Median ± interquartile range is shown. Each dot represents one donor.