Staphylococcus aureus biofilm elicits the expansion, activation and polarization of myeloid-derived suppressor cells in vivo and in vitro (original) (raw)
Fig 5
S. aureus biofilm promotes differentiation of M-MDSCs but not G-MDSCs into macrophages or M2-macrophages in vitro.
(a) Morphological characterization of the sorted M-MDSCs (CD11b+Ly-6C+Ly-6G-) and G-MDSCs (CD11b+Ly-6ClowLy-6G+). Giemsa-stained M-MDSCs (P1) and G-MDSCs (P2) were examined by light microscopy. (b) M-MDSCs and G-MDSCs were individually co-cultured with S. aureus biofilms for 72 hr. The biofilm-treated cells were subsequently analyzed for the expression of F4/80 and CD206 by flow cytometry. (c) Changes in the proportion of F4/80-positive macrophages in the sorted M-MDSCs after treatment with S. aureus biofilm (N = 3). (d) Changes in the proportion of M2-macrophages (F4/80+CD206+) in the sorted M-MDSCs after biofilm treatment (N = 3). (e and f) Treatment of G-MDSCs with S. aureus biofilm failed to affect the proportions of F4/80+ cells (macrophages) and F4/80+CD206+ cells (M2-macrophages) (N = 3). **p < 0.001.