Exosomal lncRNA GAS5 regulates the apoptosis of macrophages and vascular endothelial cells in atherosclerosis (original) (raw)
Fig 2
LncRNA GAS5 over-expressing THP-1 cells treated with 75 μg/mL oxLDL elevated cell apoptosis.
A. RT-PCR analysis of lncRNA GAS5 expression in NC and lncRNA GAS5 overexpressing cells. More than four folds of lncRNA GAS5 expression was increased in over-expressing cells. **P < 0.01, THP-1-GAS5-OE vs. NC control. B and C. Flow cytometry analysis of apoptotic THP-1 cells with lncRNA GAS5 over-expressing after treated with oxLDL (75μg/mL). **P < 0.01, THP-1-GAS5-OE vs. NC control. D. Western blot analysis of P53, Caspase 3, Caspase 7 and Caspase 9 in NC and llncRNA GAS5 overexpressing cells treated with oxLDL (75μg/mL).