A highly-sensitive high throughput assay for dynamin's basal GTPase activity (original) (raw)
Fig 1
Optimization of assay sensitivity to measure GDP production.
A) Cartoon depicting the Transcreener GDP fluorescence polarization reaction B) The GDP antibody was titrated to determine its optimal concentrations for 10, 100 and 500 μM GTP. Optimal antibody concentrations are represented by the highlighted points (n = 1, measured in triplicates). C) Standard curve representing the conversion of 0 to 100% GDP from 10 μM GTP. This curve was used to convert the fluorescence polarization data to GDP released (n = 1, measured in triplicates). Data are presented as mean ± SD.