A highly-sensitive high throughput assay for dynamin's basal GTPase activity (original) (raw)
Fig 5
Comparison of commercially available inhibitors with compound 24.
A) Dynasore and Dyngo-4a were tested alongside compound 24 in the Transcreener assay (n = 1, measured in triplicates). B) Dose response curves for the inhibitory effects of Dynasore, Dyngo-4a and compound 24 on the lipid nanotube-stimulated GTPase activity of 100 nM Dyn1 assayed in the presence of 300 μM lipid nanotubes and 25 μM GTP and measured using the malachite green assay (n = 3, each measured in triplicates). Data are presented as mean ± SD.