Citrus aurantium L. dry extracts promote C/ebpβ expression and improve adipocyte differentiation in 3T3-L1 cells (original) (raw)
Fig 1
The effects of _CA_de on TG deposition and intra-cellular lipid accumulation in 3T3-L1 adipocytes.
3T3-L1 pre-adipocytes were differentiated into mature adipocytes for 8 days, as described under “Materials and Methods”, in absence (Ctrl) or presence of _CA_de (1, 10 or 100 μg/ml). A) Total TG deposition of mature adipocytes. Data are mean ± SD of determinations from three independent experiments. Statistical analysis was performed using one-way ANOVA. **p<0.01, and ***p<0.001, vs. Ctrl. Microscopic images (10X magnification) (B), and lipid quantization (C) of mature adipocytes stained with Oil-Red O. The results are means ± SD of the Oil-Red O absorbance values measured at 490 nm from three independent experiments and are expressed as fold changes over control. Statistical analysis was performed using one-way ANOVA. **p<0.01 vs. Ctrl.