Human gut bacteria as potent class I histone deacetylase inhibitors in vitro through production of butyric acid and valeric acid (original) (raw)
Fig 4
Supernatants of gut commensals selectively inhibit HDAC Class I enzymes—Specifically HDAC2 isoform.
(A) Inhibition of different isoforms of class I (HDAC 1, HDAC2 and HDAC3) and class II (HDAC4, HDAC5, HDAC6 and HDAC9) HDACs by CFS of M. massiliensis MRx0029, R. intestinalis MRx0071, B. massiliensis MRx1342, SimMi (+/- M. massiliensis MRx0029) and dilutions of SCFA butyrate and valeric acid and MCFA hexanoic acid. (B-D) Specific inhibition of HDAC1 (B), HDAC2 (C) and HDAC3 (D) by CFS of bacterial strains M. massiliensis MRx0029, R. intestinalis MRx0071 and B. massiliensis MRx1342, SimMi (+/- M. massiliensis MRx0029) and different dilutions of SCFA butyrate and valeric acid and MCFA hexanoic acid. TSA is used as a negative control. Significances tested against YCFA * (p<0.05) ** (p<0.005) *** (P<0.001) **** (p<0.0001).