Phenolic compounds disrupt spike-mediated receptor-binding and entry of SARS-CoV-2 pseudo-virions (original) (raw)
Fig 7
Effect of selected polyphenols on internal pH and endosome acidification.
(A) Intracellular/lysosomal pH measurement. pHrodo™ Green AM dye and additional incubation for 30 min. at 37°C. Cells were then washed and fluorescence was measured at Ex/Em = 535/595 nm. Intracellular pH identification was done using standard curve prepared by measuring fluorescence in the presence of standard buffers with indicated pH as described in Material and Methods section. (B) Endosomal pH measurement in A549 cells treated with indicated polyphenols at different concentrations for 3h at 37°C. Scale bar indicates 50 μm. Images are representative of all observed fields. Experiments were done in triplicates and repeated three times. Data are presented as % of control ± SD. TF-3 –theaflavin-3,3’-digallate; control– 0.025% DMSO, positive control– 20 mM ammonia chloride.