The Beta-Glucan Receptor Dectin-1 Recognizes Specific Morphologies of Aspergillus fumigatus (original) (raw)
Figure 5
Heightened Binding of Dectin-1 to A. fumigatus SC
A soluble fusion protein consisting of the extracellular carbohydrate recognition domain of dectin-1 fused with the Fc portion of murine IgG1 (s-dectin-mFc) was constructed and incubated with live A. fumigatus RC and SC. Binding of s-dectin-mFc was detected by Cy3-conjugated, goat anti-mouse IgG antibody followed by imaging with a Zeiss Axioplan 2 upright fluorescent deconvolution microscope (Zeiss), and images were captured using 3i Slidebook Version 4.0 software (Optical Analysis). Representative micrographs show s-dectin-mFc binding to A. fumigatus grown for 2 h (A), 6 h (B), 10 h (C), and 24 h (D). Left lane images are differential interference contrast (DIC) images, and right lane images are Cy3 staining. Magnification is 630 × oil emersion for all frames.