The Beta-Glucan Receptor Dectin-1 Recognizes Specific Morphologies of Aspergillus fumigatus (original) (raw)
Figure 6
Blockage of Dectin-1-Mediated Inflammation In Vivo after A. fumigatus Lung Challenge
C57BL/6 mice were intratracheally administered live A. fumigatus conidia in the presence or absence of s-dectin-hFc. Mice were sacrificed 24 h later, and a BAL was performed.
(A) Alveolar macrophages were co-cultured for 24 h with live A. fumigatus in the presence or absence of s-dectin-hFc (10 μg/ml). Supernatant cytokine and chemokine levels were determined by Bio-Plex or ELISA. (A) illustrates cumulative results from four separate experiments. Asterisks represent significant differences between untreated and s-dectin-1–containing wells (p < 0.05). Data are expressed as mean pg/ml + SEM.
(B) Cytokine and chemokine levels in clarified BAL fluid from untreated and soluble (s-dect)–treated mice was measured by Bio-Plex. (B) illustrates representative results from three independent experiments (n = 5–7 mice per group). Asterisks represent significant differences between untreated and s-dectin-1–treated mice (p < 0.05). Data are expressed as mean pg/ml + SEM.
(C) Total cell counts (Total) in BALF fluid as enumerated on a hemacytometer. Neutrophil (PMN) concentrations were determined by calculating the percentages of neutrophils in three to five sets of 100 cells and multiplying the percentage with the total BALF cell number.
(D) Lungs were excised from non-lavaged–untreated and s-dectin-hFc–treated mice, homogenized, followed by serial 1:10 dilutions, and plated onto potato dextrose agar. CFU/lung were determined after incubating the plates for 24 h at 37 °C. (D) illustrates representative results from three independent experiments (n = 5–7 mice per group).