Identification of a Novel Gammaretrovirus in Prostate Tumors of Patients Homozygous for R462Q RNASEL Variant (original) (raw)
Figure 1
XMRV Detection by DNA Microarrays and RT-PCR
(A) Virochip hybridization patterns obtained for tumor samples from 19 patients. The samples (_x-_axis) and the 502 retroviral oligonucleotides present on the microarray (_y-_axis) were clustered using hierarchical clustering. The red color saturation indicates the magnitude of hybridization intensity.
(B) Magnified view of a selected cluster containing oligonucleotides with the strongest positive signal. Samples from patients with QQ RNASEL genotype are shown in red, and those from RQ and RR individuals as well as controls are in black.
(C) Results of nested RT-PCR specific for XMRV gag gene. Amplified gag PCR fragments along with the corresponding human GAPDH amplification controls were separated by gel electrophoresis using the same lane order as in the microarray cluster.