New Perspectives on Host-Parasite Interplay by Comparative Transcriptomic and Proteomic Analyses of Schistosoma japonicum (original) (raw)
Figure 5
Validation of Potential SNPs by RT-PCR and MS/MS Spectra
(A) The SNPs of the putative SM22.6 antigen (A12) were checked by re-sequencing of PCR products amplified from genomic DNA samples of field-collected isolates of S. japonicum obtained from five Chinese provinces, as indicated at the right. The SNPs indicated by the red arrows were identical to the findings based on EST data in this study, and the SNP sites indicated by black arrows were not identified by us, according to the stringent criteria employed. Substitutions of amino acid residues due to the missense mutations are illustrated based on the DNA sequences.
(B) An example for the verification of the translated peptide variants due to nonsynonymous SNPs by MS/MS spectra. The amino acid S (serine) of SJCHGC01743 protein was replaced here by a smaller amino acid G (glycine), where the b3 ion was shifted to the lower mass range as indicated by arrows. Both peptides were detected in the same protein extracts from female schistosomes by the mass spectrometry data. The position of the peptide within the protein sequence is highlighted with red color.