New Perspectives on Host-Parasite Interplay by Comparative Transcriptomic and Proteomic Analyses of Schistosoma japonicum (original) (raw)
Figure 6
Comparative Genomic Analysis of S. japonicum Genes with CDSs from Representative Sequenced Genomes
(A) The deduced S. japonicum proteins were compared with public protein datasets from mammalian hosts (Homo sapiens, R. norvegicus, and M. musculus), fish (T. nigroviridis and T. rubripes), insects (A. gambiae and D. melanogaster), nematodes (C. briggsae and C. elegans), protozoans (P. yoelii nigeriensis [17XNL], P. falciparum [3D7]), (Cryptosporidium parvum and C. hominis), and fungi (S. cerevisiae) at a cutoff of different BLASTP E values indicated by icons. The black bars indicated that the numbers of the deduced protein were identified by this proteomic analyses.
(B) Potential Phylum Platyhelminthes- and _Schistosoma_-specific genes were predicted through interrogating the platyhelminth and Schistosoma ESTs in GenBank. The numbers within the largest (orange) circle indicate S. japonicum CDSs that were not similar to any known protein in a GenBank dataset, which excluded all flatworm sequences at a cutoff of different BLASTP E values. The numbers within the middle-sized blue and small yellow circles represent S. japonicum CDSs with similarity to the EST data from platyhelminths and schistosomes, respectively, at a cutoff of tBLASTN E value of 10−20. The numbers within the smallest green circles indicate proteins identified by the proteomic approach.
(C) S. japonicum CDSs were compared with EST and cluster datasets of S. mansoni using the tBLASTN program at a cutoff of different E values indicated.