A Molecular Link between Malaria and Epstein–Barr Virus Reactivation (original) (raw)
Figure 4
Stimulation of Tonsil B Cells and PBMCs from EBV-Positive Donors and BL Patients with CIDR1α Increases the EBV Load
(A) Tonsil B cells were cultured in medium alone (white bar), with GST (1 μM) (light grey bar), and with CIDR1α (1 μM) (dark grey bar). After 48 h of incubation, the viral genome copy number in the cultures was determined by real-time PCR. *, p = 0,04.
(B) PBMCs from two EBV-positive healthy donors were cultured with GST (1 μM) (white bars) and with CIDR1α (1 μM) (dashed bars). After 48 h of incubation, the viral genome copy number in the cultures was determined by real-time PCR. Data are expressed as EBV copies/mL. Vertical bars depict median values, with 25th and 75th percentile values represented by the bottom and top edges of the bar. Each bar shows the EBV load from one patient measured in multiple replicates and in three independent experiments. *, _p_Donor1 = 0,04; *, _p_Donor2 = 0,001.
(C) PBMCs from children with BL were cultured with medium or GST (1 μM), our internal controls (C), and with CIDR1α (1 μM). After 48 h of incubation, the viral genome copy number in the cultures was determined by real-time PCR. Data are expressed as EBV copies/mL. Vertical bars depict median values, with 25th and 75th percentile values represented by the bottom and top edges of the bar. Each bar shows the EBV load measured in multiple replicates. *, _p_BL2 = 0,01; *, _p_BL3 = 0,01; *, _p_BL6 = 0,0003; *, _p_BL7 = 0,03.