Leishmania Manipulation of Sand Fly Feeding Behavior Results in Enhanced Transmission (original) (raw)
Figure 1
Metacyclogenesis and fPPG Synthesis in L. mexicana and L. infantum
(A) Flies were infected by membrane feeding with amastigotes of L. mexicana (filled squares) or L. infantum (filled circles), ten flies/group harvested daily, and the average number of metacyclics/fly determined. Error bars represent 1 s.e.m.
(B) Accumulation of fPPG within the midguts of infected sand flies. Midgut homogenates from flies infected with L. mexicana (upper panel) or L. infantum analyzed by SDS-PAGE and immunoblotting using AP3 mAb to detect the presence of fPPG glycans. Lane C is a day 7 uninfected blood-fed control sample.
(C) Metacyclogenesis in sand flies infected with L. mexicana exponential phase– (closed squares) or stationary phase– (half squares) cultured amastigotes. Error bars represent 1 s.e.m. Asterisks indicate statistically significant differences (p < 0.05) from stationary infections using an unpaired _t_-test.
(D) PSG accumulation in sand flies infected with L. mexicana exponential phase (upper panel) or stationary phase (lower panel) amastigotes analyzed by SDS-PAGE and immunoblotting using AP3.