Brucella Control of Dendritic Cell Maturation Is Dependent on the TIR-Containing Protein Btp1 (original) (raw)
Figure 3
B. abortus Interferes with DC Maturation
DCs were infected with different B. abortus strains or with Salmonella and labelled with the anti-MHC II and anti-LAMP1 antibodies (A) or FK2 antibody (B) to detect MVBs or DALIS, respectively.
(C) Comparison between the percentages of DCs infected with either wild-type B. abortus (black bars) or S. typhimurium (white bars) that contain DALIS. The negative control (grey bars) corresponds to mock-infected DCs with DALIS. The difference between Brucella and Salmonella infected cells at 24 h is statistically significant, p < 0.0001.
(D) Quantification of the percentage of DCs with DALIS at 24 h after incubation with media (negative), wild-type B. abortus (wt), _virB9_− mutant or heat-killed B. abortus (HK). The differences between wild-type wt and either _virB9_− or HK are statistically significant (p = 0.0059 and p = 0.018, respectively). For (C) and (D) data represent means ± standard errors of at least three independent experiments. Bars: 10 μm (A); 5 μm (B).