Salp15 Binding to DC-SIGN Inhibits Cytokine Expression by Impairing both Nucleosome Remodeling and mRNA Stabilization (original) (raw)
Figure 6
Salp15 Does Not Affect DC Maturation, but Dose-Dependently Inhibits IL-12p70, IL-6, and TNF-α Production by DCs Stimulated with Viable Borrelia burgdorferi
(A) Salp15 does not interfere with viable B. burgdorferi-induced maturation of DCs. Immature DCs were either not stimulated (dotted line), or stimulated with 1 × 10 5 or 1 ×10 6 of viable B. burgdorferi in the absence (solid line) or presence (bold line) of Salp15. The grey-filled graphs represent isotype controls. B. burgdorferi was preincubated with Salp15 for 30 min before addition of DCs. 18 h post-stimulation DCs were analysed by FACS for surface expression of CD80 and CD86.
(B) Salp15 inhibits viable B. burgdorferi-induced pro-inflammatory cytokine production by DCs. The experiment was performed as in (A). However, for this assay supernatant was analyzed for cytokine production by CBA.
The experiments were performed with DCs from four independent donors. Bars represent the mean of triplicates within one experiment ± SE. Differences in cytokine production between DCs stimulated with B. burgdorferi and DCs stimulated with B. burgdorferi and Salp15 were analyzed by a two-sided one way ANOVA, implementing a one way analysis of variance with Dunnett multiple comparison test. A _p_-value < 0.05 was considered statistically significant, indicated as one asterisk (*) 0.01 < p < 0.05 or two asterisks (**) 0.001 < p < 0.01.