agr-Mediated Dispersal of Staphylococcus aureus Biofilms (original) (raw)
Figure 9
Effect of a serine protease inhibitor and protease deficient mutants on AIP-I mediated biofilm detachment.
Columns show CLSM reconstructions of biofilms at day 2, day 3 and day 4. Biofilms were grown for 2 days and the growth media was supplemented with AIP-I or AIP-I+PMSF as indicated. Greenish yellow color indicates expression of the agr P3-GFP reporter, and the red color is from propidium iodide present in the growth medium. (A) Wild type biofilm (AH462) supplemented with 50 nM AIP-I. (B) Wild type biofilm (AH462) supplemented with 50 nM AIP-I and 10 µM PMSF. (C) Aureolysin (Δ_aur_) mutant biofilm (AH789) supplemented with 50 nM AIP-I. (D) Aureolysin Spl (Δ_aur_ Δ_spl_) double mutant biofilm (AH788) supplemented with 50 nM AIP-I. CSLM reconstructions are representative of three separate experiments and each side of a grid square represents 20 µM. Percent biomass detached was calculated by COMSTAT analysis comparing biomass at day 2 to biomass at day 4.