A Sporozoite Asparagine-Rich Protein Controls Initiation of Plasmodium Liver Stage Development (original) (raw)
Figure 5
Remodelling of the PVM is impaired in _SLARP_-deficient parasites.
(A) HepG2 cells were infected with WT or slarp(-) sporozoites in the presence of FITC-dextran and cultured for 6 hours before staining with anti-CSP (green), anti-UIS4 (red) and filipin (blue), followed by analysis by confocal fluorescence microscopy. Bar = 5 µm. Note that sporozoites inside dextran-positive cells were not stained by anti-UIS4 or filipin. (B) The percentage of UIS4 and filipin stained sporozoites was determined by examination of at least 50 dextran-negative infected cells for each population. (C) HepG2 cells were infected with WT or slarp(-) sporozoites and cultured for 48 hours before staining with anti-HSP70 (green) and anti-EXP1 (red) antibodies and analysis by confocal fluorescence microscopy. Bar = 10 µm. (D) The percentage of HSP70 and EXP1 labelled parasites was determined by examination of at least 50 GFP-positive 48h LS for each population.