Ameobal Pathogen Mimivirus Infects Macrophages through Phagocytosis (original) (raw)
Figure 3
APMV particles colocalize with clathrin, but not with caveolin-1.
(A) RAW 264.7 macrophages overexpressing GFP-caveolin-1 (GFP-Cav1) were incubated with APMV particles (50 PFU/cell) for 15 min. Viral particles were visualized by indirect immunofluorescence and laser scanning microscopy. The lack of colocalization with GFP-caveolin-1 was demonstrated by merging fluorescent images. (B) RAW 264.7 macrophages were incubated with APMV particles (50 PFU/cell) for 1 hour. The colocalization of viral particles with Alexa 488-conjugated clathrin antibodies was determined. Merged images showed the colocalization of APMV particles with clathrin. Inset confirms the colocalization of clathrin with viral particles. (C) RAW 264.7 macrophages were incubated with APMV particles (50 PFU/cell) for different periods. The number of APMV particles that colocalized with clathrin was scored. The results are expressed as the percentage of AMPV particles that colocalized with clathrin and are the mean±SD of 4 experiments. Scale bars represent 3 µm.