Ameobal Pathogen Mimivirus Infects Macrophages through Phagocytosis (original) (raw)
Figure 9
Role of the PI3K pathway in APMV internalization.
(A) RAW 264.7 Macrophages were pretreated with different concentrations of LY294002 for 30 min and incubated with APMV particles for 6 hours in the presence of LY294002. Viral particles were visualized by immunofluorescence. The results, expressed as the percentage of APMV uptake relative to the control, are the mean±SD of 3 experiments. (B) Immunoblotting of macrophages stimulated with APMV particles (50 PFU/cell) for different periods was performed with antibodies specific for phosphorylated Akt and ERK. Membranes were stripped and reprobed with anti-Akt and -ERK antibodies, respectively. Each blot is representative of three distinct experiments.