Ameobal Pathogen Mimivirus Infects Macrophages through Phagocytosis (original) (raw)
Figure 12
Role of dynamin-II in M. avium internalization.
RAW 264.7 macrophages (A), macrophages transiently transfected with GFP (B), GFP-tagged active (C) or dominant-negative (D) dynamin-II were infected with Texas red-labelled M. avium (10 bacteria/cell) for 6 hours. Fluorescent organisms were visualized by epifluorescence (middle panels). Bacteria were not associated with macrophages transfected with the dominant-negative mutant of dynamin-II. (E) The number of internalized bacteria was scored. The results, expressed as the percentage of bacterial uptake relative to the control, are the mean±SD of 4 experiments. Scale bars represent 25 µm. *p<0.05.