Molecular Ecology and Natural History of Simian Foamy Virus Infection in Wild-Living Chimpanzees (original) (raw)
Figure 2
Detection of SFVcpz antibodies in chimpanzee fecal samples.
Enhanced chemiluminescent (ECL) Western blot analysis of fecal extracts from (A) human volunteers and captive chimpanzees, and (B) SFVcpz infected wild chimpanzees representing four different chimpanzee subspecies. Strips were prepared using an infectious molecular clone (pMod-1) of SFVcpz_Pts_ (see Methods). Samples are numbered, with letters indicating the species (panel A) or collection site (panel B) of origin. Molecular weights of SFVcpz specific Gag and Bet proteins are shown. The banding pattern of plasma from an SFVcpz infected chimpanzee (used at a 1∶100,000 dilution) and an uninfected human are shown as positive (Pos) and negative (Neg) controls, respectively.