Commensal-Induced Regulatory T Cells Mediate Protection against Pathogen-Stimulated NF-κB Activation (original) (raw)
Figure 4
Cytokine production is suppressed in _S. typhimurium_-infected mice when pre-treated with B. infantis.
Cytokine release by isolated cells was examined immediately prior to S. typhimurium infection (Day 0) or four days (Day 4) after infection. (A) In vitro cytokine production by anti-CD3/CD28 stimulated Peyer's patch cells is significantly reduced in S. typhimurium infected mice when fed B. infantis with no differences being observed prior to infection; (B) In vitro IFN-γ and IL-10 production by anti-CD3/CD28 stimulated spleenocytes is significantly increased in placebo-fed animals following S. typhimurium infection. However, this increase was not observed in animals consuming B. infantis. TNF-α levels were similar for the two groups; (C) LPS stimulated splenocytes released comparable amounts of cytokine from the two groups of animals prior to infection but four days following Salmonella translocation, LPS stimulated TNF-α, IL-6 and MCP-1 release was significantly less in the _B. infantis_-fed animals. *p<0.05 versus placebo, n = 8/group at each timepoint.