Commensal-Induced Regulatory T Cells Mediate Protection against Pathogen-Stimulated NF-κB Activation (original) (raw)
Figure 7
NF-κB activation & TNF-α secretion in response to LPS is significantly reduced by adoptive transfer of CD4+CD25+ T cells from _B. infantis_-fed animals.
(A) In vivo adoptive transfer of CD4+ NF-κB−/− T cells from _B. infantis_-fed animals resulted in significant attenuation of NF-κB activity following i.p. LPS administration in NF-κBlux+/+ animals compared to mice that received T cells from placebo-fed controls (n = 6/group); (B) A subsequent study performed an identical adoptive transfer experiment except that nuclear NF-κB activation was measured using an ELISA system instead of biophotonic imaging - identical results were observed in that CD4+ T cells from _B. infantis_-fed animals significantly reduced LPS stimulated NF-κB activation (n = 5/group); (C) Removal of the CD25+ subpopulation from the CD4+ cells resulted in loss of the NF-kB suppressive activity while adoptive transfer of the CD25+ population alone replicated the CD4+ suppressive effect; (D) Similarily, the reduction in TNF-α secretion associated with CD4+ T cell transfer was lost when CD25+ were depleted but was replicated with transfer of CD25+ cells alone (n = 5/group). *p<0.05 versus placebo T cells; **p<0.05 versus CD4 T cells.