High-Resolution Functional Profiling of Hepatitis C Virus Genome (original) (raw)
Figure 2
Electropherogram depicting the location of 15-nt insertions in p7-NS2 region and the mutant population replication dynamics during selection.
Each peak (X-axis) represents the location of a 15-nt insertion in the p7-NS2 region, and the fluorescent signal intensity (Y-axis) indicates the abundance of each 15-nt insertion mutant. The number at the top of the figure corresponds to the JFH-1 genome position of the p7-NS2 region. The electropherogram panels show the insertion profile of the mutant plasmid library (DNA input), in vitro transcribed RNA library (RNA input), and Huh-7.5.1 cell culture selected mutant viral library [selection 2, 4, 10, 16, and 21 days post-transfection (dpt)]. The complexity of the library is similar in DNA input, RNA input, 2 dpt and 4 dpt. Most of the 15-nt insertion mutants have been negatively selected at 10 dpt. Note that the HCV mutants containing 15-nt insertions around p7-NS2 junctions have shown strong positive selection. Asterisks indicate an artifact peak generated during data processing. To better visualize the short peaks, the fluorescent signal intensity scale was set at 2000; hence some of the tall peaks are shown out of scale.