High-Resolution Functional Profiling of Hepatitis C Virus Genome (original) (raw)
Figure 5
Functional Profiles of the JFH-1 HCV 5BSL3 CRE and 3′NTR _cis_-elements.
The stem loop structures are shown. The colors of the filled circles represent the phenotypes: lethal (red), attenuating (blue), and tolerated (green). Insertions at 5BSL- 3.1 and 3.2 were lethal for virus replication. Many insertions at the bulge region between CRE 5BSL- 3.2 and 3.3 were tolerated. The kissing-loop interaction between CRE 5BSL3.2, and 3′SL2 is depicted with dotted lines. The 3′NTR predicted variable region stem loop structures (VSL1 and VSL2), poly (U/UC) tract, and 3′X tail stem loop structures are shown. Many insertions at VSL2 were tolerated and attenuating. The stop codon UAG is highlighted. All the insertions at poly-U tract were lethal.